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Determination of Gallic Acid in Rat Plasma by LC-MS-MS |
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| Authors: | Song Rui Xu Lei Zhang Zunjian Tian Yuan Xu Fengguo Dong Haijuan |
| Institution: | 1.Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, 210009, China ;2.Center for Instrumental Analysis, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, China ; |
| Abstract: | Liquid chromatography–electrospray ionization tandem mass spectrometry has been used for rapid, selective, and sensitive quantitative analysis of gallic acid in rat plasma. Sample pretreatment involved a one-step extraction using ethyl acetate with protocatechic acid as internal standard. Separation was on a C18 column using an isocratic mobile phase, consisting of methanol-0.1% aqueous formic acid (40:60, v/v) at 0.2 mL min−1. The stability of gallic acid was evaluated in acidified and non-acidified plasma. The method was validated then successfully applied to a pharmacokinetic study in rats after oral administration of rhubarb extract. |
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