Reproducibility and variation of diffusion measures in the squirrel monkey brain,in vivo and ex vivo |
| |
| Institution: | 1. Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, TN, USA;2. Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA;3. Department of Psychology, Vanderbilt University, Nashville, TN, USA;4. Department of Electrical Engineering, Vanderbilt University, Nashville, TN, USA;1. Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA;2. Department of Industrial and Systems Engineering, University of Minnesota, Minneapolis, MN 55455, USA;3. Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA;4. Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;1. Department of Radiology, Baoji Center Hospital, Baoji, Shaanxi, People''s Republic of China;2. Department of Radiology, Zhengzhou University First Affiliated Hospital, Zhengzhou, Henan, People''s Republic of China;1. Department of Radiology, Wayne State University, Detroit, MI 48201;2. The MRI Institute for Biomedical Research, 761 Lucerne Avenue, Waterloo, ON, Canada;3. Department of Physics, Wayne State University, Detroit, MI 48201;4. Department of Neurology, Henry Ford Health System, Detroit, MI 48202 |
| |
| Abstract: | PurposeAnimal models are needed to better understand the relationship between diffusion MRI (dMRI) and the underlying tissue microstructure. One promising model for validation studies is the common squirrel monkey, Saimiri sciureus. This study aims to determine (1) the reproducibility of in vivo diffusion measures both within and between subjects; (2) the agreement between in vivo and ex vivo data acquired from the same specimen and (3) normal diffusion values and their variation across brain regions.MethodsData were acquired from three healthy squirrel monkeys, each imaged twice in vivo and once ex vivo. Reproducibility of fractional anisotropy (FA), mean diffusivity (MD), and principal eigenvector (PEV) was assessed, and normal values were determined both in vivo and ex vivo.ResultsThe calculated coefficients of variation (CVs) for both intra-subject and inter-subject MD were below 10% (low variability) while FA had a wider range of CVs, 2–14% intra-subject (moderate variability), and 3–31% inter-subject (high variability). MD in ex vivo tissue was lower than in vivo (30%–50% decrease), while FA values increased in all regions (30–39% increase). The mode of angular differences between in vivo and ex vivo PEVs was 12 degrees.ConclusionThis study characterizes the diffusion properties of the squirrel monkey brain and serves as the groundwork for using the squirrel monkey, both in vivo and ex vivo, as a model for diffusion MRI studies. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
