Determination of onapristone and its N-desmethyl metabolite in human plasma or serum by high-performance liquid chromatography |
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Authors: | C Zurth F Kagels |
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Affiliation: | Schering AG, Pharmacokinetics, Berlin, F.R.G. |
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Abstract: | An automated reversed-phase high-performance liquid chromatographic method for the determination of the antiprogestin onapristone and its N-desmethyl metabolite in human plasma or serum is described. Ultraviolet detection was performed at 315 nm, with a limit of detection of 1 ng/ml at a signal-to-noise ratio of 3. The intra- and inter-assay precision were less than or equal to 6% and less than or equal to 7%, respectively. Onapristone and its N-desmethyl metabolite were stable in human plasma. The method was successfully applied to serum samples obtained from human volunteers after oral administration of onapristone. |
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