超高效液相色谱-串联质谱法检测花生中36种农药及其代谢物残留

建立了花生中36种农药及其代谢物残留的超高效液相色谱-串联质谱(UHPLC-MS/MS)快速检测技术。采用乙腈提取,增强型脂质去除净化剂(EMR-Lipid)净化,正离子多反应监测(MRM)模式测定。结果表明,所有农药的线性相关系数均大于0.994,在0.005,0.01,0.10 mg/kg 3个加标水平下,36种农药的平均回收率为70.4%~119%,相对标准偏差(RSDs)为1.3%~19.4%,方法的定量下限为0.002 5~0.05 mg/kg。该方法简便、快速,灵敏度高、净化效果好,适用于花生中农药多残留的快速检测分析。

Rapid Detection of 36 Pesticide Residues in Peanut by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

A multiresidue analytical method was developed for the rapid detection of 36 pesticides in peanut using ultra high performance liquid chromatography-tandem mass spectrometric technique(UHPLC-MS/MS).Peanut samples were extracted with acetonitrile,and then cleaned up with QuEChERS EMR-Lipid.The extract was detected by UHPLC-MS/MS.The positive ion mode and multiple reaction monitoring(MRM) mode were used to identify and quantify 36 pesticide residues in peanut.All pesticides had good linearity with correlation coefficients above 0.994.The average recoveries of the 36 pesticides ranged from 70.4% to 119% with relative standard deviations(RSDs) of 1.3%-19.4% at spiked levels of 0.005,0.01,0.10 mg/kg.The quantitation limits of this method were in the range of 0.002 5-0.05 mg/kg. With the advantages of simplicity,rapidness,sensitivity and good purifying effect,the method was suitable for the rapid determination of pesticide residues in peanut.