Modifications to the NIST reference measurement procedure (RMP) for the determination of serum glucose by isotope dilution gas chromatography/mass spectrometry |
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Authors: | Jocelyn L Prendergast Lorna T Sniegoski Michael J Welch Karen W Phinney |
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Institution: | (1) Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8392, USA |
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Abstract: | The definitive method (DM), now known as the reference measurement procedure (RMP), for the analysis of glucose in serum was
originally published in 1982 by the National Institute of Standards and Technology (NIST). Over the years the method has been
subject to a number of modifications to adapt to newer technologies and simplify sample preparation. We discuss here an adaptation
of the method associated with serum glucose measurements using a modified isotope dilution gas chromatography/mass spectrometry
(ID-GC/MS) method. NIST has used this modified method to certify the concentrations of glucose in SRM 965b, Glucose in Frozen
Human Serum, and SRM 1950, Metabolites in Human Plasma. Comparison of results from the revised method with certified values
for existing Standard Reference Materials (SRMs) demonstrated that these modifications have not affected the quality of the
measurements, giving both good precision and accuracy, while reducing the sample preparation time by a day and a half. |
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