基于大环多胺的新型线型聚合物的合成及与DNA相互作用的研究

设计合成了一种新型线形大环聚合多胺（POGEC），该聚合物可通过1，3-丙二醇二缩水甘油基醚和1，7-二（二乙氧磷酰基）-1，4，7，10-四氮杂环十二烷反应得到．琼脂糖凝胶电泳和电子扫描光谱（SEM）显示POGEC与DNA有很强的结合能力．当生理浓度的氯化钠溶液加入到已经形成的POGEC／DNA复合物中，DNA可被有效释放．通过荧光光谱测量了POGEC与DNA的结合能力及紧缩能力．圆二色光谱表明POGEC的强结合力并未改变DNA的构象．

Novel cyclen-based linear polymer as a high-affinity binding material for dna condensation

A novel cyclen-based linear polyamine (POGEC) was designed and synthesized from the reaction between 1,3-propanediol diglycidyl ether and 1,7-bis(diethoxyphosphory)-1,4,7,10-tetraazacyclododecane. High-affinity binding between POGEC and DNA was demonstrated by agarose gel electrophoresis and scanning electron micros-copy (SEM). Moreover, the formed POGEC/DNA complex (termed polyplex) can be disassociated to release the free DNA through the addition of physiological concentration of NaCl solution. Fluorescen...