| 高效液相色谱法测定人参单体皂甙的研究──醋酸铵对人参皂甙分离的色谱改性 |
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| 引用本文： | 顾光华,周蕾,赵贵文.高效液相色谱法测定人参单体皂甙的研究──醋酸铵对人参皂甙分离的色谱改性[J].色谱,1994,12(1):40-42. |
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| 作者姓名： | 顾光华 周蕾 赵贵文 |
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| 作者单位： | 中国科学技术大学，上海市环境监测中心水质室，广州化学所 |
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| 基金项目： | 国家自然科学基金与高校博士学科点专项科研基金资助课题 |
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| 摘 要： | 讨论了醋酸铵对高效液相色谱法（ＨＰＬＣ）分离人参单体皂甙的色谱改性情况，经试验选择采用ＭＰＧ－ＯＤＳ色谱柱，ＵＶ２０４ｎｍ处检测，以乙腈－水－醋酸铵为流动相，在１５分钟内较好地分离了单体皂甙Ｒ＿（ｇ２）、Ｒ＿（ｂ１）、Ｒ＿ｃ、Ｒ＿ｄ、Ｒ＿（ｇ１）、Ｒ＿ｅ等。方法操作简单、快速，可用于人参质量评定的常规分析。
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| 关 键 词： | 醋酸铵 反相高效液相色谱 改性剂 流动相 人参单体皂甙R＿（g2）、R＿（b1）、R＿c、R＿d、R＿（g1）、R＿e. |
Improved High Performance Liquid Chromatographic(HPLC)Methodfor the Analysis of Ginsenosides in Panax Ginseng Extracts & Products the Chromatographic Modifier Action of Ammonium Acetate in Separation of Ginsenosides |
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| Gu Guanghua,Zhou Lei and Zhao Guiwen.Improved High Performance Liquid Chromatographic(HPLC)Methodfor the Analysis of Ginsenosides in Panax Ginseng Extracts & Products the Chromatographic Modifier Action of Ammonium Acetate in Separation of Ginsenosides[J].Chinese Journal of Chromatography,1994,12(1):40-42. |
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| Authors: | Gu Guanghua Zhou Lei and Zhao Guiwen |
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| Abstract: | he chromatographic properties of ammonium acetate in HPLC mobile phase for ginsenosides assay havebeen studied, and six ginsenosides(R_(g2),T_(b1),R_c,R_d,R_(g1),R_e)were separated by the method using a column ofoctadecylsilyl chemically modified porous glass(MPG-ODS)within 15 minutes and eluted with acetonitrile-waterammonium acetate with UV detector at 204nm.The ammonium acetate in this eluant improved the separatingdegree of ginsenosides in crude drugs.This method is simple,rapid and accurate,It can be used for assaying ginseng raw materials and ginsengpreparatlons et al. |
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| Keywords: | RP-HPLC mobile phase modifier ammonium acetate ginsenosides R_(g2) R_(b1) R_c R_d R_(g1) R_e |
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