蒽醌及黄酮类化合物与牛血清白蛋白结合的反应的光谱研究

用停留技术研究了大黄素与牛血清白蛋白(BSA)结合反应的动力学.发现在25℃,pH10.0时结合在场100ms即可达到平衡.大黄素等7种蒽醌及黄酮类化合物与BSA 结合后,可见区最大吸收波长红移.吸收强度增加. 荧光光谱研究表明. 这些化合物对BSA荧光有很强的猝灭作用.根据猝灭结果, 求出了它们与BSA 的结合常数, 并基于Forster非辐射能量转移机理,计算了它们的第一结合部位与BSA中212- 色氨酸残基的距离.提出了蒽醌及黄酮类化合物与BSA形成复合物的结构模型.

Spectroscopic studies ob Binding of anthraquinones and fiavonoids to bovine serum albumin

Stopped flow studies how that the binding equilibrium of emodin to bovine serum albumin (BSA) is established within 100 ms. The adsorption max. of these compounds exhibit red shift upon binding and the absorbance enhanced. The fluorescence quenching associated with the binding of these compounds to BSA was used to measure binding constants, and the distance of Trp-212 to the first binding site were calculated, on the basis of the energy transfer between BSA and bound complexes. An allosteric domain model of emodin-BSA complex was postulated.