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基于牛血清白蛋白荧光探针的盐酸特拉唑嗪含量测定
引用本文:季仁东,赵志敏,张吉华,陈梦岚,朱星玥. 基于牛血清白蛋白荧光探针的盐酸特拉唑嗪含量测定[J]. 发光学报, 2014, 35(8): 1014-1017. DOI: 10.3788/fgxb20143508.1014
作者姓名:季仁东  赵志敏  张吉华  陈梦岚  朱星玥
作者单位:1. 南京航空航天大学 理学院, 江苏 南京 210016;2. 淮阴工学院 电子与电气工程学院, 江苏 淮安 223003
基金项目:国家自然科学基金(10722043); 教育部博士点基金(20093218110024); 江苏省普通高校研究生科研创新计划(CXLX12_0143); 南京航空航天大学博士学位论文创新与创优基金(BCXJ12-10); 中央高校基本科研业务费专项资金; 淮安市科技支撑社会发展指导性项目(HASZ2013007)资助
摘    要:对牛血清白蛋白(BSA)与盐酸特拉唑嗪(THD)混合体系进行了荧光发射光谱和同步光谱实验研究。BSA溶液在340 nm处有明显荧光特征峰,当加入THD后导致牛血清白蛋白发生内源荧光猝灭。据此建立了以BSA为探针的THD含量测定方法,通过该方法所得的模型函数相关系数都高于0.99。对所得模型函数进 行了实验验证,其中发射光谱模型函数回收率为96.04%~103.16%,同步光谱模型函数回收率为100.41%~ 105.58%,相对标准偏差分别为3.70%和2.42%。荧光发射光谱和同步光谱方法的检出限分别为0.64×10-8 mol/L和0.66×10-8 mol/L,定量限分别为0.21×10-7 mol/L和0.22×10-7 mol/L。

关 键 词:牛血清白蛋白  盐酸特拉唑嗪  荧光光谱  含量检测
收稿时间:2014-05-13

Determination of Terazosin Hydrochloride by Using Bovine Serum Albumin as Fluorescent Probe
JI Ren-dong,ZHAO Zhi-min,ZHANG Ji-hua,CHEN Meng-lan,ZHU Xing-yue. Determination of Terazosin Hydrochloride by Using Bovine Serum Albumin as Fluorescent Probe[J]. Chinese Journal of Luminescence, 2014, 35(8): 1014-1017. DOI: 10.3788/fgxb20143508.1014
Authors:JI Ren-dong  ZHAO Zhi-min  ZHANG Ji-hua  CHEN Meng-lan  ZHU Xing-yue
Affiliation:1. College of Science, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China;2. Faculty of Electronic and Electrical Engineering, Huaiyin Institute of Technology, Huaian 223003, China
Abstract:The fluorescence emission and synchronous spectra of the bovine serum albumin (BSA) and terazosin hydrochloride (THD) mixed system were studied. The most intensive characteristic peak (340 nm) was found in the spectrum of BSA solution, and the addition of THD resulted in the fluorescence quenching of BSA. Based on this phenomenon, the determination method for THD was established, in which BSA was used as the fluorescent probe. The correlation coefficients of the model functions are all more than 0.99. The recoveries of emission and synchronous spectra are 96.04%~103.16% and 100.41%~105.58%, the relative standard deviations (RSD) are 3.70% and 2.42%, the limits of detection are 0.64×10-8 mol/L and 0.66×10-8 mol/L, and the limits of quantification are 0.21×10-7 mol/L and 0.22×10-7 mol/L, respectively.
Keywords:bovine serum albumin  terazosin hydrochloride  fluorescence spectrum  content determination
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