荧光光谱结合校正算法测定混合色素溶液中各组分的浓度

应用英国Edinburgh公司生产的FLS920P荧光光谱仪实验测定了诱惑红、日落黄和亮蓝三种合成食品色素混合溶液的三维荧光光谱，将荧光光谱数据应用化学计量学中的平行因子分析(PARAFAC)和交替三线性分解(ATLD)二阶校正算法进行计算处理，对混合合成食品色素溶液中各组分进行了定性和定量检测。应用核一致诊断法，确定主成分数为3。PARAFAC算法解析后的回收率分别为98.75%±8.9%，97.22%±2.9%和99.00%±2.9%，ATLD算法解析后的回收率分别为99.78%±5.9%，92.52%±5.5%和97.23%±5.8%。结果表明，两种方法都可以用于三个组分的直接快速测定，PARAFAC算法更稳定，更具优势。

Determination of Components of Mixed Color Solutions by Fluorescence Spectroscopy Combined with Calibration Algorithms

The three-dimensional spectra of mixed solutions of allure red, sunset yellow and brilliant blue were obtained. Then the three synthetic food colors were determined by parallel factor analysis (PARAFAC) and alternating trilinear decomposition (ATLD) algorithms, respectively. The component number of model is three by core-consistency diagnostic. The average recoveries of allure red, sunset yellow and brilliant blue obtained by PARAFAC were 98.75%±8.9%, 97.22%±2.9% and 99.00%±2.9% and those by ATLD algorithm were 99.78%±5.9%, 92.52%±5.5% and 97.23%±5.8%, respectively. Results show that both of the algorithms can be used in direct and rapid determination of multi-components of mixtures. From further comparison, the PARAFAC is more stable and advantageous.