Compact polytetrafluoroethylene assembly-type capillary electrophoresis with chemiluminescence detection |
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Authors: | Tsukagoshi Kazuhiko Ishida Shingo Oda Yuichi Noda Keiichi Nakajima Riichiro |
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Institution: | Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University, Kyotanabe 610-0321, Japan. ktsukago@mail.doshisha.ac.jp |
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Abstract: | We have developed a compact polytetrafluoroethylene (PTFE) assembly-type capillary electrophoresis with chemiluminescence (CL) detection system. Luminol-microperoxidase-hydrogen peroxide chemiluminescence reaction was adopted. The device is rectangular in shape (60 mm x 40 mm x 30 mm) and includes three reservoirs (sample, migration buffer, and detection reservoirs) with electrodes. The detection reservoir includes an optical fiber to transport light at the capillary tip to a photomultiplier tube. Isoluminol isothiocyanate (ILITC) was analyzed as a model using this device with fused-silica or polytetrafluoroethylene capillary tubes 10 cm in length. We also used the sample reservoir as a reactor for an immune reaction between anti-human serum albumin immobilized on glass beads and isoluminol isothiocyanate-labeled human serum albumin. The present polytetrafluoroethylene assembly with the capillary tube was useful as a palm-sized analysis device for separation and detection, as well as a reactor. |
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