电解质溶液组成对低分子量阴离子毛细管电泳分离的影响

研究了毛细管电泳间接紫外检测法测定低分子量阴离子时电解质溶液中背景电解质、电渗流改性剂、ｐＨ值、有机溶剂等对分离的影响；比较了铬酸根、邻苯二甲酸根、苯甲酸根３种背景离子对不同迁移率阴离子分离的影响，并对间接紫外检测的定量基础及灵敏度进行了讨论；考察了３种不同长链烷基三甲基季铵盐电渗流改性剂浓度对阴离子迁移时间和电渗迁移率的影响，结果表明电渗流的改性效果与烷基链的长度有关；ｐＨ影响阴离子的有效迁移率

Effects of Carrier Electrolyte Composition on Capillary Electrophoretic Separation of Low Molecular Mass Anions

A systematic investigation was carried out on the effects of carrier electrolyte composition on separation in capillary electrophoresis of low molecular mass anions with in direct UV detection, including background electrolytes, electroosmotic flow modifiers, pH and organic solvent. Chromate, phthalate or benzoate was used as background electrolyte. The separation efficiency depended on the mobility match between the analyte and background ions. The quantitative aspects and the sensitivity of indirect UV detection were discussed. The influences of electroosmotic flow modifier on migration time and electroosmotic rate of anions were investigated using three different long-chain alkyltrimethyl quaternary ammonium salts. The ability of electroosmotic flow modification depended on the length of alkyl chain. Organic solvent affected the migration time and the selectivity of separation to some extent. The results are useful for the optimization of capillary electrophoretic separation of low molecular mass anions.