| 寡糖-8-氨基芘-1,3,6-三磺酸衍生物的毛细管电泳-激光诱导荧光分离 |
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| 引用本文： | 党福全,陈义,郭晴,徐桂芸.寡糖-8-氨基芘-1,3,6-三磺酸衍生物的毛细管电泳-激光诱导荧光分离[J].高等学校化学学报,2000,21(2):206. |
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| 作者姓名： | 党福全 陈义 郭晴 徐桂芸 |
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| 作者单位： | 中国科学院化学研究所分子科学中心, 北京 100080 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，中国科学院重大项目 |
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| 摘 要： | 利用自组装的毛细管电泳-激光诱导荧光装置,研究了多种寡糖-8-氨基芘-1,3,6-三磺酸(寡糖-APTS)衍生物的分离.考察了电泳介质、浓度及pH对寡糖-APTS衍生物分离的影响,在酸性和碱性条件下,分别实现了痕量寡糖标准品及葡聚糖水解产物的高效分离
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| 关 键 词： | APTS 寡糖 葡聚糖 毛细管电泳-激光诱导荧光 |
| 收稿时间： | 1999-07-06 |
Ultrasensitive Fluorometric Detection of Oligosaccharides as Their APTS Derivatives by Capillary Electrophoresis |
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| DANG Fu-Quan,CHEN Yi,GUO Qing,XU Gui-Yun.Ultrasensitive Fluorometric Detection of Oligosaccharides as Their APTS Derivatives by Capillary Electrophoresis[J].Chemical Research In Chinese Universities,2000,21(2):206. |
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| Authors: | DANG Fu-Quan CHEN Yi GUO Qing XU Gui-Yun |
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| Institution: | Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China |
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| Abstract: | Reducing oligosaccharides, after reductive amination, were separated and detected as their APTSderivatives by capillary electrophoresis with laser-induced fluorescence. Brief optimization studies have also been performed with respect to CEseparation conditions of oligosaccharides. The oligosaccharide ladders were analyzed in an acidic phosphate-tris buffer(pH2.5) or in the alkaline borate buffer. Acid hydrolysis products of dextran T-40 labeled with APTSwere also successfully mapped. |
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| Keywords: | 8-Aminopyrene-1 3 6-trisufonate(APTS) Oligosaccharide Dextran Capillary electropho-resis with laser-induced fluorescence detection |
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