Selective Enrichment of a Methanol-Utilizing Consortium Using Pulp and Paper Mill Waste Streams |
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Authors: | Gregory R Mockos William A Smith Frank J Loge David N Thompson |
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Institution: | (1) Department of Civil and Environmental Engineering, University of California at Davis, 1 Shields Avenue, Davis, CA 95616, USA;(2) Biological Sciences Department, Idaho National Laboratory, P.O. Box 1625, Idaho Falls, ID 83415-2203, USA |
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Abstract: | Efficient utilization of carbon inputs is critical to the economic viability of the current forest products sector. Input
carbon losses occur in various locations within a pulp mill, including losses as volatile organics and wastewater. Opportunities
exist to capture this carbon in the form of value-added products such as biodegradable polymers. Waste-activated sludge from
a pulp mill wastewater facility was enriched for 80 days for a methanol-utilizing consortium with the goal of using this consortium
to produce biopolymers from methanol-rich pulp mill waste streams. Five enrichment conditions were utilized: three high-methanol
streams from the kraft mill foul condensate system, one methanol-amended stream from the mill wastewater plant, and one methanol-only
enrichment. Enrichment reactors were operated aerobically in sequencing batch mode at neutral pH and 25°C with a hydraulic
residence time and a solids retention time of 4 days. Non-enriched waste activated sludge did not consume methanol or reduce
chemical oxygen demand. With enrichment, however, the chemical oxygen demand reduction over 24-h feed/decant cycles ranged
from 79 to 89%, and methanol concentrations dropped below method detection limits. Neither the non-enriched waste-activated
sludge nor any of the enrichment cultures accumulated polyhydroxyalkanoates (PHAs) under conditions of nitrogen sufficiency.
Similarly, the non-enriched waste activated sludge did not accumulate PHAs under nitrogen-limited conditions. By contrast,
enriched cultures accumulated PHAs to nearly 14% on a dry weight basis under nitrogen-limited conditions. This indicates that
selectively enriched pulp mill waste activated sludge can serve as an inoculum for PHA production from methanol-rich pulp
mill effluents. |
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Keywords: | Foul condensate Waste-activated sludge Polyhydroxyalkanoates Pulp mill Natural fiber reinforced thermoplastic composite |
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