| CdS纳米粒子与半胱氨酸相互作用的研究 |
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| 引用本文： | 赵书林,沈江珊. CdS纳米粒子与半胱氨酸相互作用的研究[J]. 高等学校化学学报, 2005, 26(9): 1613-1617 |
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| 作者姓名： | 赵书林 沈江珊 |
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| 作者单位： | 南开大学化学系,天津,300071;南开大学化学系,天津,300071;南开大学化学系,天津,300071;南开大学化学系,天津,300071;南开大学化学系,天津,300071;南开大学化学系,天津,300071 |
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| 基金项目： | 国家自然科学基金;广西自然科学基金 |
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| 摘 要： | 合成了粒径均匀和分散性好的CdS纳米粒子.通过改变CdS纳米粒子及半胱氨酸的浓度、体系的pH值及CdCl2和CH3CSNH2摩尔比等实验条件跟踪监测了CdS纳米粒子光谱性质的变化,探讨了CdS纳米粒子与半胱氨酸之间的相互作用及化学反应机理.
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| 关 键 词： | CdS纳米粒子 半胱氨酸 荧光光谱 |
| 文章编号： | 0251-0790（2005）09-1613-05 |
| 收稿时间： | 2004-09-08 |
| 修稿时间： | 2004-09-08 |
Analysis of D-Aspartic Acid and D-Glutamic Acid in Single Cells by Capillary Electrophoresis |
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| ZHAO Shu-Lin,SHEN Jiang-Shan. Analysis of D-Aspartic Acid and D-Glutamic Acid in Single Cells by Capillary Electrophoresis[J]. Chemical Research In Chinese Universities, 2005, 26(9): 1613-1617 |
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| Authors: | ZHAO Shu-Lin SHEN Jiang-Shan |
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| Affiliation: | College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China |
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| Abstract: | An analytical method was established to detect D-aspartic acid(D-Asp) and D-glutamic acid(D-Glu) in single cells. Cellular samples were derivatized with a fluorescence tagging reagent, naphthalene-2,2-dicarboxaldehyde in a mini-volume(3 μL) prior to the separation. Sixteen pair of amino acid enantiomers resolution was achieved by using a dual chiral selector system consisting of β-cyclodextrin(β-CD) and chiral micelles formed by sodium deoxycholate(SDC). The separation was coupled with laser induced fluorescence detection. Single neurons isolated from the ganglia of Aplysia were analyzed to determine the D/(D+L) molar ratios of Asp to Glu. D-Asp and D-Glu were found at high levels and its cellular distribution was highly heterogeneous. |
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| Keywords: | D-Aspartic acid D-Glutamic acid Chiral separation Single cells analysis Capillary electrophoresis |
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