HPLC法同时测定石蒜中加兰他敏和石蒜碱

建立了高效液相色谱同时测定石蒜中加兰他敏和石蒜碱含量的方法。 采用phenomenex-C18色谱柱，以乙腈(A)-0.1%TFA水溶液(B)为流动相梯度洗脱，检测波长289 nm。 结果表明，加兰他敏和石蒜碱均在0.5～10 mg/L(r=0.9999)呈现良好的线性关系，最低检测限(S/N=3)分别为0.09和0.15 mg/L，平均加样回收率分别为99.53%和96.82%。 该方法简单、快速、准确，适合于石蒜中加兰他敏和石蒜碱的测定。

Determination of Galanthamine and Lycorine in Lycoris Herb. by HPLC

A high performance liquid chromatographic method was developed for simultaneous determining the contents of galanthamine and lycorine in Lycoris Herb. The chromatographic analysis was carried out on a phenomenex-C18 column. The mobile phase was acetonitrile(A)-0.1%trifluoroacetic(TFA)(B) with gradient elution. The detection wavelength was set at 289 nm. The calibration curves for galanthamine and lycorine showed good linearity in the range of 0.5～10 mg/L(r=0.9999). The detection limits were 0.09 mg/L for galanthamine and 0.15 mg/L for lycorine at the signal-to-noise ratio of 3, and the average recoveries were 99.53% and 96.82%, respectively. The method is simple, rapid, accurate and suitable for the determination of galanthamine and lycorine in Lycoris Herb.