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| 甜茶叶中甜茶苷的UPLC-MS/MS分析 | |
| 引用本文： | 张健,侴桂新,章苏宁,刘李明,刘志军,宋小秋. 甜茶叶中甜茶苷的UPLC-MS/MS分析[J]. 光谱实验室, 2011, 28(5): 2692-2696 |
| 作者姓名： | 张健 侴桂新 章苏宁 刘李明 刘志军 宋小秋 |
| 作者单位： | 1. 上海应用技术学院香料香精技术与工程学院 上海市漕宝路120号200235;上海中医药大学中药研究所 上海市蔡伦路1200号201203 2. 上海中医药大学中药研究所 上海市蔡伦路1200号201203 3. 上海应用技术学院香料香精技术与工程学院 上海市漕宝路120号200235 4. 美国路易斯安娜州立大学自然资源学院 美国路易斯安娜州巴吞鲁日城70803 |
| 基金项目： | 上海应用技术学院重点项目资助(KJ2008-16) |
| 摘 要： | 采用超高效液相色谱-质谱联用法测定广西特产甜茶叶中的主要甜味成分甜茶苷的含量.样品经前处理后以Acquity UPLC BEH C18柱为色谱柱,流动相为乙腈(含体积分数为0.1％甲酸)-蒸馏水(含体积分数0.1％甲酸),梯度条件为10min乙腈相体积由20％变化到65％,柱温35℃,进样量5μL,流速为0.15mL/... |
| 关 键 词： | 甜茶苷 超高效液相色谱-质谱联用 甜茶叶 |
Determination of Rubusoside from Rubus Suavissimus S.Lee Leaves by UPLC-MS/MS |
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| ZHANG Jian,CHOU Gui-Xin,ZHANG Su-Ning,LIU Li-Ming,LIU Zhi-Jun,SONG Xiao-Qiu. Determination of Rubusoside from Rubus Suavissimus S.Lee Leaves by UPLC-MS/MS[J]. Chinese Journal of Spectroscopy Laboratory, 2011, 28(5): 2692-2696 | |
| Authors: | ZHANG Jian CHOU Gui-Xin ZHANG Su-Ning LIU Li-Ming LIU Zhi-Jun SONG Xiao-Qiu |
| Affiliation: | ZHANG Jiana,b CHOU Gui-Xinb ZHANG Su-Ninga LIU Li-Minga LIU Zhi-Junc SONG Xiao-Qiuaa(School of Perfume and Aroma Technology,Shanghai Institute of Technology,Shanghai,200235,P.R.China)b(Institute of Chinese Material Medicine,Shanghai University of Traditional Medical School,201203,P.R.China)c(School of Renewable Natural Resources,Louisiana State University,Baton Rouge,LA 70803 U.S.A.) |
| Abstract: | A method for determination of rubusoside in sweet tea leaves was established by ultra performance liquid chromatography electrospray ionization mass spectrometry（UPLC-ESI MS/MS）.After pretreatment procedure,sample was separated on a Acquity UPLC BEH C18 column with A acetonitrile（with 0.1% formic acid,V/V） and B distilled water（with 0.1% formic acid,V/V）as mobile phase.The gradient elution was from 0 to 10 min followed a linear change from 20% to 65%（V/V）for phase A with injection volume of 5μL at a flow rate of 0.15 mL/min.The column temperature was set at 35℃.Detection was performed by multiple reaction monitoring（MRM） on mass spectrometer.Electrospray ionization source was applied and operated in the positive ion mode（ESI＋）,and m/Z 643→319 was selecting as detecting ion.A good linear calibration was obtained in the range of 0.1—10.0μg/mL with correlation coefficient of 0.9996.The average addition standard recovery was 96.6%.The LOD and LOQ were 0.04μg/mL and 0.1μg/mL.The proposed method is specific,sensitive and reproducible enough to be used for the determination of rubusoside in sweet tea leaves. |
| Keywords: | Rubusoside UPLC-MS/MS Rubus Suavissimus S.Lee Leaves |
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