| 超高效液相色谱-质谱法测定人体血浆中的环磷酰胺 |
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| 引用本文： | 王文辉,杨俊,陈瑶,王齐,孙晓东,朱艳琴.超高效液相色谱-质谱法测定人体血浆中的环磷酰胺[J].光谱实验室,2011,28(6):2887-2891. |
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| 作者姓名： | 王文辉 杨俊 陈瑶 王齐 孙晓东 朱艳琴 |
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| 作者单位： | 1. 昆明理工大学分析测试研究中心 昆明市学府路253号 650093
2. 云南省第一人民医院 昆明市金碧路157号650051 |
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| 基金项目： | 云南省科技厅应用基础基金(20092C171M) |
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| 摘 要： | 建立了测定人体血浆中环磷酰胺血药浓度的超高效液相色谱-串联质谱方法.样品经甲醇沉淀蛋白,高速离心分离提取,用Waters Acquity UPLC BEH C18色谱柱(50mm×2.1mm,1.7μm)分离,以0.1％甲酸水溶液∶乙腈=(80∶20)为流动相进行洗脱,流速0.4mL·min-1,采用电喷雾质谱正离子模...
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| 关 键 词： | 超高效液相色谱-质谱法 人体血浆 环磷酰胺 |
Determination of Cyclophosphamide in Human Plasma by Ultra Performance Liquid Chromatography-Mass Spectrometry |
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| WANG Wen-Hui,YANG Jun,CHEN Yao,WANG Qi,SUN Xiao-Dong,ZHU Yan-Qin.Determination of Cyclophosphamide in Human Plasma by Ultra Performance Liquid Chromatography-Mass Spectrometry[J].Chinese Journal of Spectroscopy Laboratory,2011,28(6):2887-2891. |
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| Authors: | WANG Wen-Hui YANG Jun CHEN Yao WANG Qi SUN Xiao-Dong ZHU Yan-Qin |
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| Institution: | WANG Wen-Hui YANG Jun CHEN Yaoa WANG Qi SUN Xiao-Dong ZHU Yan-Qin(Research Center for Analysis and Measurement,Kuming University of Science and Technology,Kunming 650093,P.R.China)a(First People' s Hospital of Yunnan Province,Kunming 650051,P.R.China) |
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| Abstract: | The method for the determination of the content of cyclophosphamide in human plasma was established by ultra performance liquid chromatography-mass spectrometry（UPLC/MS/MS）method.The samples were extracted by high-speed centrifugation and protein precipitation with methanol.The separation of cyclophosphamide was performed on Waters Acquity UPLC BEH C18 column（50mm×2.1mm,1.7μm） with the mobile phase of 0.1% ethanoic acid aqueous solution∶acetonitrile（80∶20） at a flow rate of 0.4mL·min-1,and that were determined by positive ESI MS/MS.The detection daughter ions of MS/MS were m/z 140.0 and 106.0 by external standard method.The calibration curves were good linear between the concentrations of 2—100ng·mL-1（r=0.9999）.The limit of detection was 1μg·L-1.The recoveies of cyclophosphamide were in the range of 98.4%—100.5% and the RSD was less than 2.5%.This method is suitable for the study on clinical pharmacokinetics of cyclophosphamide. |
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| Keywords: | UPLC/MS/MS Human Plasma Cyclophosphamide |
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