|
|||||
|
|
| 高效液相色谱法测定溴吡斯的明白蛋白微球药物含量 | |
| 引用本文： | 李艺,谭群友,蒋心惠,赵春景,张景勍.高效液相色谱法测定溴吡斯的明白蛋白微球药物含量[J].光谱实验室,2011,28(5):2646-2650. |
| 作者姓名： | 李艺 谭群友 蒋心惠 赵春景 张景勍 |
| 作者单位： | 1. 重庆医科大学药物高校工程研究中心和生物化学与分子药理学重点实验室 重庆市渝中区医学院路1号400016 2. 第三军医大学大坪医院野战外科研究所胸外科中心 重庆市渝中区长江支路10号400042 3. 重庆医科大学附属第二医院药剂科 重庆市临江路76号400010 |
| 基金项目： | 教育部博士点基金资助项目(20095503120008); 重庆市教育委员会资助项目(首批高等学校优秀人才资助,KJ090308); 第三军医大学临床课题(2007XG062) |
| 摘 要： | 采用Hypersil ODS2 (4.6mm×250mm,5μm)色谱柱；以0.01mol/L庚烷磺酸钠溶液(磷酸二氢钠0.01mol/L,用10％磷酸调节pH=3.0)∶乙腈(77∶23)为流动相；紫外检测波长269nm；流速1.0mL/min；柱温为30℃.溴吡斯的明在5.75-57.5μg/mL浓度范围内线性关系... |
| 关 键 词： | 溴吡斯的明 白蛋白微球 高效液相色谱法 |
Determination of Pyridostigmine Bromide in Bovine Serum Ablum Microspheres by HPLC |
|
| LI Yi,TAN Qun-You,JIANG Xin-Hui,ZHAO Chun-Jing,ZHANG Jing-Qing.Determination of Pyridostigmine Bromide in Bovine Serum Ablum Microspheres by HPLC[J].Chinese Journal of Spectroscopy Laboratory,2011,28(5):2646-2650. | |
| Authors: | LI Yi TAN Qun-You JIANG Xin-Hui ZHAO Chun-Jing ZHANG Jing-Qing |
| Institution: | LI Yi TAN Qun-Youa JIANG Xin-Hui ZHAO Chun-Jingb ZHANG Jing-Qing(Medicine Engineering Research Center,Chongqing Key Laboratory of Biochemical & Molecular Pharmacology,Chongqing Medical University,Chongqing 400016,P.R.China)a(Center of Thoracic Surgery,Daping Hospital & Research Institute of Surgery,Third Military Medical University,Chongqing 400042,P.R.China)b(Pharmacy Department,The Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,P.R.China) |
| Abstract: | The method for the determination of pyridostigmine bromide in bovine serum ablum microspheres was established by HPLC,with Hypersil ODS2（4.6mm×250mm,5μm）as chromatographic column,and 0.01mol/L heptane-1-sulfonic acid sodium salt solution（pH was adjusted to 3.0 by 10% phosphoric acid）：acetonitrile（77∶23） as mobile phase.The detection wavelength was 269nm,and flow rate was 1.0mL/min with the column of 30℃.The linear range of pyridostigmine bromide was in the range of 5.75—57.5μg/mL,and the linear regression equation was A=66.907C-12.583（r=0.9998）.The average recovery of oyridostigmine bromide was 99.90%,and RSD was 1.85%.The method is accurate,simple and highly specificity,that applied to the determination of pyridostigmine bromide in bovine serum ablum microspheres. |
| Keywords: | Pyridostigmine Bromide Album Microspheres HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |