| 化学发光法测定尿液中8羟-基脱氧鸟苷 |
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| 引用本文： | 颜流水,熊萌瑶,郭会琴,任艳,黄燕. 化学发光法测定尿液中8羟-基脱氧鸟苷[J]. 光谱实验室, 2011, 28(6): 2784-2787 |
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| 作者姓名： | 颜流水 熊萌瑶 郭会琴 任艳 黄燕 |
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| 作者单位： | 南昌航空大学环境与化学工程学院 南昌市丰和南大道696号330063 |
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| 基金项目： | 国家自然科学基金资助项目(20765003) |
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| 摘 要： | 利用8-羟基脱氧鸟苷（8-OHdG）在酸性邻菲咯啉（Phen）-Cu化学发光体系中发光信号强的特点,建立了一种测定尿样中8-OHdG含量的方法。本方法的线性范围为1.67—333.33μg/L,检出限（S/N=3）为0.83μg/L。对167.00μg/L的8-OHdG平行测定11次,其相对标准偏差为1.2%。该法用于尿液中8-OHdG的测定,方法简便、灵敏、快速,分析结果令人满意。
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| 关 键 词： | 化学发光 8羟-基脱氧鸟苷 固相萃取 尿样 |
Detection of 8-Hydroxydeoxyguanosine in Urine Samples by Chemiluminescence Method |
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| YAN Liu-Shui,XIONG Meng-Yao,GUO Hui-Qin,REN Yan,HUANG Yan. Detection of 8-Hydroxydeoxyguanosine in Urine Samples by Chemiluminescence Method[J]. Chinese Journal of Spectroscopy Laboratory, 2011, 28(6): 2784-2787 |
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| Authors: | YAN Liu-Shui XIONG Meng-Yao GUO Hui-Qin REN Yan HUANG Yan |
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| Affiliation: | YAN Liu-Shui XIONG Meng-Yao GUO Hui-Qin REN Yan HUANG Yan(College of Environment and Chemical Engineering,Nanchang Hangkong University,Nanchang 330063,P.R.China) |
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| Abstract: | A chemiluminescence（CL） method was developed for determination of 8-OHdG for its characteristics of strong light signal in acid phenanthroline（Phen）-Cu CL system.The CL intensity was linear with the concentration of 8-OHdG in the range of 1.67—333.33μg/L under the optimum experimental conditions.The detection limit（S/N=3） was 0.83μg/L,and the relative standard deviation（RSD） was 1.2% for 167.00μg/L 8-OHdG（n=11）.The method for determination of urinary 8-OHdG is simple,sensitive,rapid and satisfactory. |
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| Keywords: | Chemiluminescence 8-Hydroxydeoxyguanosine Solid Phase Extraction Urine Samples |
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