| 间接法测定吴茱萸碱磷脂复合物的复合率 |
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| 引用本文： | 王红,谭群友,徐美玲,吴建勇,张景勍.间接法测定吴茱萸碱磷脂复合物的复合率[J].光谱实验室,2011,28(5):2608-2611. |
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| 作者姓名： | 王红 谭群友 徐美玲 吴建勇 张景勍 |
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| 作者单位： | 1. 重庆医科大学高校药物工程研究中心和生物化学与分子药理重点实验室 重庆市渝中区医学院路1号400016
2. 第三军医大学大坪医院野战外科研究所胸外科 重庆市渝中区长江支路10号400042 |
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| 基金项目： | 国家自然科学基金项目(30973645); 重庆市教育委员会资助项目(首批高等学校优秀人才资助,KJ090308) |
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| 摘 要： | 建立吴茱萸碱磷脂复合物复合率的测定方法。采用紫外分光光度法测定游离的吴茱萸碱的含量,从而计算吴茱萸碱磷脂复合物的复合率。结果表明,吴茱萸碱在225nm处有最大吸收,浓度在1.05—4.20μg.mL-1范围内与吸光度呈良好的线性关系,回归方程为：A=0.1776C＋0.006,r=0.9997;平均加标回收率为99.71%,RSD为1.26%（n=9）。该法操作简便、精密度好、结果准确可靠。
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| 关 键 词： | 吴茱萸碱 磷脂复合物 复合率 |
Determination of Recombination Rate of Evodiamine-Phospholipid Complex by Indirect Method |
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| WANG Hong,TAN Qun-You,XU Mei-Ling,WU Jian-Yong,ZHANG Jing-Qing.Determination of Recombination Rate of Evodiamine-Phospholipid Complex by Indirect Method[J].Chinese Journal of Spectroscopy Laboratory,2011,28(5):2608-2611. |
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| Authors: | WANG Hong TAN Qun-You XU Mei-Ling WU Jian-Yong ZHANG Jing-Qing |
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| Institution: | WANG Hong TAN Qun-Youa XU Mei-Ling WU Jian-Yong ZHANG Jing-Qing(Medicine Engineering Research Center,Chongqing Key Laboratory of Biochemical&Molecular Pharmacology,Chongqing Medical University,Chongqing 400016,P.R.China)a(Thoracic Surgery Center,Daping Hospital & Research Institute of Surgery,Third Military Medical University,Chongqing 400042,P.R.China) |
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| Abstract: | The method for the determination of recombination rate of evodiamine-phospholipids complex was established.The content of free evodiamine was determined by UV spectrophotometry,and the recombination rate of evodiamine-phospholipids complex was calculated.The evodiamine had the maximum absorptance at 225nm,and had a good linear relationship in the range of 1.05—4.20μg·mL-1 with absorbance.The regression equation was A=0.1776C＋0.006,r=0.9997;the average recovery was 99.71%,and RSD was 1.26%（n=9）.This method has simple operation,good precision,and the results are accurate and reproducible. |
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| Keywords: | Evodiamine Phospholipid Complex Recombination rate |
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