| 急性子中总皂苷的含量测定 |
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| 引用本文： | 王巍,鞠成国,刘威,宋爽,黄晓晨.急性子中总皂苷的含量测定[J].光谱实验室,2011,28(6):3029-3032. |
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| 作者姓名： | 王巍 鞠成国 刘威 宋爽 黄晓晨 |
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| 作者单位： | 辽宁中医药大学药学院 辽宁省大连开发区双D港生命一路77号116600 |
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| 摘 要： | 采用香草醛-冰醋酸-高氯酸显色法,以齐墩果酸为对照,分光光度法测定急性子中总皂苷的含量.经显色后,齐墩果酸在541nm处有最大吸收,并在7.327-25.64μg/mL范围内吸光度与齐墩果酸量呈良好线性关系(r=0.9996),方法平均回收率为98.7％(n=6),RSD为0.51％.该方法简便、准确、灵敏,可以快速测...
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| 关 键 词： | 急性子 总皂苷 齐墩果酸 分光光度法 |
Determination of Total Saponins in the Seeds of Impatiens Balsamina L. |
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| WANG Wei,JU Cheng-Guo,LIU Wei,SONG Shuang,HUANG Xiao-Chen.Determination of Total Saponins in the Seeds of Impatiens Balsamina L.[J].Chinese Journal of Spectroscopy Laboratory,2011,28(6):3029-3032. |
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| Authors: | WANG Wei JU Cheng-Guo LIU Wei SONG Shuang HUANG Xiao-Chen |
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| Institution: | WANG Wei JU Cheng-Guo LIU Wei SONG Shuang HUANG Xiao-Chen(School of Pharmacy,Liaoning University of TCM,Dalian,Liaoning 116600,P.R.China) |
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| Abstract: | The content of total saponins in the seeds of Impatiens balsamina L.was determined by spectrophotometry at 541nm with oleanolic acid as reference substance and colorating with vanillin-glacial acetic acid-perchloric acid.The calibration curve was linear in the range of 7.327—25.64μg/mL（r=0.9996）.The average recovery of oleanolic acid was 98.7%（RSD=0.51%,n=6）.The method is simple,accurate,sensitive.It can be applied to the quality control of the seeds of Impatiens balsamina L.. |
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| Keywords: | Seeds of Impatiens Balsamina L Total Saponins Oleanolic Acid Spectrophotometry |
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