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Low-frequency dynamics of DNA in Brillouin light scattering spectra |
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| Authors: | S G Lushnikov A V Dmitriev A I Fedoseev G A Zakharov A V Zhuravlev A V Medvedeva B F Schegolev E V Savvateeva-Popova |
| Institution: | 1. Ioffe Physical Technical Institute, Russian Academy of Sciences, St. Petersburg, 194021, Russia 2. St. Petersburg State University, St. Petersburg, 199034, Russia 3. Pavlov Institute of Physiology, St. Petersburg, 199034, Russia |
| Abstract: | Brillouin light scattering studies of deoxyribonucleic acid (DNA) in the temperature interval 297–375 K are presented. The DNA fragment (119 bp) from the first intron of D melanogaster limk1 gene with AT-rich insertion (28 bp, mutant agn ts3) was used as an experimental sample. The temperature dependence of the hypersonic velocity was found to exhibit anomalies in the vicinity of 347 and 335 K. Computer modeling of possible conformational states which might be attained by the DNA fragment under study has shown the existence of local structures that evolve with varying temperature. Combined analysis of experimental data and results of the modeling reveals a close relation between the anomalous behavior of Brillouin light scattering spectra and conformational DNA dynamics. The results are discussed in the framework of modern models of conformational DNA transformations. |
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