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Crystallization and Preliminary Crystallographic Studies of Luffaculin 1, a Ribosome-inactivating Protein from the Seeds of Luffa Acutangula |
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| Authors: | HOU Xiao-Min CHEN Ming-Huang XIE Jie-Ming YE Xiao-Ming ZHAO Geng-Xiang YANG Feng CHEN Li-Qing HUANG Ming-Dong |
| Affiliation: | 1. State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, The Chinese Academy of Sciences, Fuzhou, Fujian 350002, China;Graduate School of the Chinese Academy of Sciences, Beijing 10039, China 2. State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, The Chinese Academy of Sciences, Fuzhou, Fujian 350002, China 3. Fujian Medical University, Fuzhou 350004, China 4. Laboratory for Structural Biology, Department of Chemistry, Graduate Programs of Biotechnology,Chemistry and Materials Science, University of Alabama in Huntsville, Huntsville, AL 35899, USA |
| Abstract: | Luffaculin 1 purified from the seeds of Luffa acutangula belongs to the type 1 ribosome-inactivating proteins (RIPs). It has been crystallized by the vapor-diffusion method using polyethylene glycol as a precipitant. The crystal is of space group P1 with a = 39.135, b = 46.813,c= 83.571 (A), α = 89.068, β = 80.009 and γ = 72.143°, and has two molecules per asymmetric unit.X-ray data have been collected to be 1.4 (A) using a synchrotron source. |
| Keywords: | Luffa acutangula luffaculin 1 ribosome-inactivating proteins crystallization |
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