Interface-Free Coupling Of Hplc And Atomic Spectrometry For Trace Metal Speciation By Using A High Performance Flow And Nebulization System |
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| Authors: | G Weber H Berndt |
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| Institution: | Institut für Spektrochemie und angewandte Spektroskopie , Bunsen-Kirchhoff-Str. 11, W-4600, Dortmund 1, Germany |
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| Abstract: | Abstract The on-line coupling of HPLC with flame-AAS using a HPF/HHPN system (high performance flow/hydraulic high pressure nebulization) is described. The proposed system is used for speciation of trace metals. High molecular weight species (protein-bound metals) as well as low molecular weight ones (organic acid complexes of metals) are separated and specifically detected. Problems arising from the flow-rate and composition of the mobile phase are discussed and it is shown that these problems can be solved by optimizing the HHP-nebulizer. |
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| Keywords: | HPLC on-line AAS detection metal speciation hydraulic high pressure nebulization |
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