Synthesis of dirhodium[3H]tetraacetate |
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Authors: | R. P. Singhal Y. Sarwar |
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Affiliation: | (1) Chemistry Department, Wichita State University, 67208 Wichita, KS, USA |
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Abstract: | Realizing a need for labeled dirhodium tetraacetate [Rh2/OAc/4] for binding studies by the equilibrium dialysis procedure, we have established a method for the preparation and purification of [3H]Rh2/OAc/4. This is obtained with a tritium label of a sufficiently high specific activity in the methyl group of acetate residues. The method consists of replacement of the existing acetate bridge by [3H]OAc–. A typical preparation involves refluxing Rh2/OAc/4 with [3H]NaOAc in dry ethanol at 80°C for 6 h. The reaction time is critical for obtaining the product with a high specific activity. A simple purification of reaction products yields the compound of interest with a high specific activity, i. e., 315±10 Ci per mol of Rh2/OAc/4 and in satisfactory yield /87% of the starting material., This labeled material can be synthesized with greatly increased specific activity /of tritium in acetate groups/ by employing [3H]NaOAc without prior dilution. The labeled Rh2/OAc/4 is stable and yields a characteristic product with adenylic acid. |
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