| 早期流产组织冰冻切片中胚胎成分的分离检验 |
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| 引用本文： | 李艮平,李彩霞,郝建文,胡兰,万立华,韩俊平,杨帆.早期流产组织冰冻切片中胚胎成分的分离检验[J].四川激光,2012(3):79-81. |
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| 作者姓名： | 李艮平 李彩霞 郝建文 胡兰 万立华 韩俊平 杨帆 |
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| 作者单位： | [1]重庆医科大学,重庆400016 [2]公安部物证鉴定中心,北京100038 [3]去医遗传学公安部重点实验室,北京100038 [4]重庆市公安局,重庆400016 [5]中国人民公安大学,北京100038 |
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| 摘 要： | 目的：建立激光捕获显微切割技术与低体积扩增技术联用，进行冰冻切片上胚胎与母体成分DNA的分离检验技术。方法：制备早期流产组织冰冻切片，通过激光捕获显微切割胚胎绒毛细胞，设置10，20，30个细胞数目梯度，比较各组胚胎成分DNA的STR分型的检出率、等位基因丢失率和非特异性扩增情况。最后进行实际样本应用。结果：捕获10个胚胎绒毛细胞即可获得Identifier试剂完整的STR分型。冰冻切片捕获30个细胞数目组的检出率较高，等位基因丢失率和非特异性扩增率较低。结论：激光捕获显微切割技术联用低体积扩增技术可应用于胚胎与母体成分DNA的精确分离和检验。
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| 关 键 词： | 法医物证学 流产绒毛 冰冻切片 单细胞分离检验技术 |
Separation and testing of Chorionic Villi Isolated from frozen section slides abortion material |
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| LI Gen - ping,LI Cai - xia,HAO Jian - wen,HU Lan,WAN Li - hua,HAN Jun - ping,YANG Fan.Separation and testing of Chorionic Villi Isolated from frozen section slides abortion material[J].Laser Journal,2012(3):79-81. |
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| Authors: | LI Gen - ping LI Cai - xia HAO Jian - wen HU Lan WAN Li - hua HAN Jun - ping YANG Fan |
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| Institution: | 1. Chongqing Medical University, Chongqing 400016, China; 2. Institute of Forensic Science of Ministry of Public Security, Beijing 100038, China; 3. Key Laboratory of Forensic Genetics, Ministry of Public Secm-ity, Beijing 100038, China 4. Chongqing Public Security Bureau, Chongqing 400016, China; 5. Chinese Peoples＇ Public University, Beijing 100038, China) |
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| Abstract: | Objective： To establish a technology platform, using single- cell separation technique and LCM to realize accurate separation and testing fetal and maternal tissue in mixture of samples~ Method： Frozen section slides were prepared, setting the number of gradient cells 10,20, 30 , then, the artificial alleles, detection rate and allelie dropout rate of each group were compared. The technology was applied into an actual case. Result：Capturing 10 cells of villus can obtain the fetal STR - DNA type. The detection rate of 30 cells is high. Artificial alleles and allelie dropout rate are low. Conclusion： Single - cell separation technique combined with LCM can be applied to accurately separate and test mixture of fetal and maternal tissue, so as to improve evidence value of mixed biological samoles. |
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| Keywords: | forensic genetic chorionic villi frozen section slide single- cell separation technique |
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