人参皂苷Rg1协同rhBMP-2对人牙髓干细胞成牙分化作用研究

目的：探讨不同浓度人参皂苷Rg1、重组骨形态发生蛋白2（rhBMP-2）及两者联合应用对体外培养的人牙髓干细胞（DPSCs）成牙分化的影响。方法：酶消化法获得DPSCs，采用碱性磷酸酶（ALP）活性检测和实时荧光定量RT—PCR测定牙本质涎磷蛋白1（DSPP）和牙本质基质蛋白1（DMP1）mRNA的表达以检测不同浓度人参皂苷Rgt（0．1，0．5，2．5，5，10，20μmol／L）及rhBMP-2（25，50，100，200rig／ha）单独及联合应用对DPSCs成牙分化的影响。结果：人参皂苷Rg1（2．5，5和10μmol／L）组的ALP活性明显高于同期对照组（P〈0．001），5μmol／L组ALP活性明显高于其它浓度组（P〈0．001）；浓度为50，100，200mg/ml的rhBMP-2其ALP活性明显高于同期对照组（P〈0．001），100ng／ml rhBMP-2组ALP活性最强。联合应用人参皂苷Rg1（5μmol／L）和rhBMP-2（100ng／ml）DSPP和DMP1 mRNA的表达明显高于单独应用组（P〈0．001）。结论：人参皂苷Rg1可促进体外培养的DPSCs成牙本质分化，人参皂苷：Rg1协同rhBMP-2能明显增强DISCs成牙本质分化。

Effect of ginsenoside Rgl and recombinant bone morphogenetic protein 2 on differentiation of human dental pulp stem cells in vitro

Objective： The aim of the present study is to evaluate the effect of ginsenoside Rgl and recombinant bone morphogen-etic protein 2（rhBMP- 2） on the differentiation of human dental pulp stem cells （DPSCs）. Methods： DPSCs were isolated and cultured. The cells were incubated with different concentrations of ginseneside Rgl （ O. 1, 0.5, 2.5, 5, 10, 20/maol/L} and BaBMP - 2 [ 25, 50,100 和 200 ng/ml ）. The effect on the differentiation of DPSCs was assayed by ALP enzymatic activity, and the gene expression of dentin sialophesphoprotein（DSPP） and dentin matrix protein 1 （DMP1 ） by real time RT - PCR. Results： Comparing with the control group, ALP activity was sigaaificantly enhanced in ginseneside P, gl （2.5, 5, and 10μmol/L} group（P 〈 0. 001）, especially in ginsenoside Rgl （5tanol/L} group. ALP activity was significantly enhanced in rhBMP- 2 （ 50, 100,200 ng/ml ） group, especially in daBMP- 2 （100 ng/ml） group. The gene expression of DSPP and DMP1 were significantly increased in the combination group of glnsenoside Rgl （5μmol/L} and rhBMP- 2 （ 100 ng/ml ） compared with single application of ginsenoside Rgl or rhBMP - 2 only at the same time points （ P 〈 0.001）. Conclusion： The results indicate that ginsenoside Rgl promotes the differentiation of DPSCs. Combined application of ginsenoside Rgl and rhBMP2 can greatly promote DPSCs differentiation.