| 白眉蝮蛇蛇毒和蛇毒酶的基质辅助激光解吸电离飞行时间质谱法研究 |
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| 引用本文： | 季怡萍,郭锬,孙明忠,刘淑莹.白眉蝮蛇蛇毒和蛇毒酶的基质辅助激光解吸电离飞行时间质谱法研究[J].分析测试学报,2000,19(6):81-83. |
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| 作者姓名： | 季怡萍 郭锬 孙明忠 刘淑莹 |
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| 作者单位： | 中国科学院长春应用化学研究所,吉林,长春,130022 |
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| 摘 要： | 应用基质辅激光解吸电离飞行时间质谱（ＭＡＬＤＩ－ＴＯＦ－ＭＳ）法对长白山眉蝮蛇蛇毒和纯化得到的两种蛇毒酶进行了研究,得到了它们的分子质量并验证了纯度。同时还考察了不同产地的蛇毒、蛇毒蛋白浓度以及基质对分析结果的影响,实验结果表明ＭＡＬＤＩ－ＴＯＦ－ＭＳ法是检测蛋白纯化过程和分析蛋白相对分子质量十分有效的手段。
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| 关 键 词： | 白眉蝮蛇 蛇毒 蛇毒酶 MALDI－TOF－MS |
MALDI- TOF- MS Analysis of Venom and Venom Enzymes of Agkistrodon Blomhoffii Ussurensis |
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| JI Yi-ping,GUO Tan,SUN Ming-zong,LIU Shu-ying.MALDI- TOF- MS Analysis of Venom and Venom Enzymes of Agkistrodon Blomhoffii Ussurensis[J].Journal of Instrumental Analysis,2000,19(6):81-83. |
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| Authors: | JI Yi-ping GUO Tan SUN Ming-zong LIU Shu-ying |
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| Abstract: | The venom and two purified venom enzymes of Agkistrodon blomhoffii Ussuresis have been studied by MALDI - TOF - MS. The relative molecular masses of venom enzymes were determined and their purity tested. Effects of the haunt of snakeS, venom protein concentrations and substrate concen- trations were studied. The results indicate that MALSI - TOF - MS is one of the most effective tools for the assessment of protein purification processes and for the determination of protein molecular masses. |
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| Keywords: | Agkistrodon blomhoffii Ussurensis Venom Snake venom enzyme MALDI - TOF - MS |
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