Preparation of radiolabeled clonidine for use in radioimmunoassay |
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| Authors: | H L Bhalla R Mathew Grace Samuel N Sivaprasad |
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| Institution: | (1) B. V. Patel PERD Centre, Thaltej, 380 054 Ahmedabad, India;(2) Khandelwal Lab. Ltd., 400 033 Bombay, India;(3) Isotope Division, Bhabha Atomic Research Centre, 400 085 Bombay, India;(4) Radiopharmaceuticals Operations, BRIT, 400 705 New Bombay, India |
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| Abstract: | The preparation of stable and immunoreactive radioiodinated clonidine for the development of a radioimmunoassay of clonidine is described. Tyrosylmethyl ester (TME) conjugate of carboxylic acid derivative of clonidine was radioiodinated using chloramine T as the oxidizing agent. The radioiodinated clonidine was purified by thin layer chromatography, gel filtration and solvent extraction. Radiochemical purity, specific activity and immunoreactivity were determined. The stability of the labeled compound during storage was tested in various solvents at different temperatures. |
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