| 用宿主范围扩大的重组AcNPV在家蚕中表达TNF |
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| 引用本文： | 胡敏,刘映乐,齐义鹏,朱应.用宿主范围扩大的重组AcNPV在家蚕中表达TNF[J].武汉大学学报(理学版),2001,47(6):732-736. |
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| 作者姓名： | 胡敏 刘映乐 齐义鹏 朱应 |
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| 作者单位： | 武汉大学病毒研究所, |
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| 基金项目： | 国家863高技术新探索项目(39880031) |
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| 摘 要： | 用宿主范围扩大的核型多角体病毒双穿梭载体Bonpvid与重组转座质粒 pFast-TNF转座，使含有有TNF基因的表达盒插入Bonpvid的Tn7att的接受位点筛选阳性重组E.coli菌落，将提取的阳性重组子DNA分别转染家蚕BmN细胞和Sf-9细胞，获得了能表达TNF的重组病毒，SDS－PAGE和Western blot分析表明，TNF在上述两种细胞中得到表达，以家蚕作为生物反应器，将含有重组病毒的的细胞上清注射感染家蚕4龄幼虫，使TNF在蚕体表达，用BmN细胞中表达的TNF感梁肿瘤细胞，诱导细胞发生了凋亡。
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| 关 键 词： | 肿瘤坏死因子（TNF）－α 宿主范围扩大 转基因动物 重组病毒 AcNPV家蚕 重组转座质粒 基因表达 |
| 文章编号： | 0253-9888(2001)06-0732-05 |
| 修稿时间： | 2001年6月28日 |
Expression of Tumor Necrosis Factor in Bombyx mori by Host Range Expanded Recombinant AcNPV |
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| HU Min,LIU Ying-le,QI Yi-peng,ZHU Ying.Expression of Tumor Necrosis Factor in Bombyx mori by Host Range Expanded Recombinant AcNPV[J].JOurnal of Wuhan University:Natural Science Edition,2001,47(6):732-736. |
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| Authors: | HU Min LIU Ying-le QI Yi-peng ZHU Ying |
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| Abstract: | Recombinant virus vAcBmT was obtained after transposition of pFast-TNF into Tn7 att in bonpvid which is a host range expanded Bi-shuttle vector by Bac-to-Bac system.vAcBmT DNA transfected BmN cell and Sf-9 cell respectively, SDS-PAGE and Western blot showed that the TNF was expressed in both of the cells. The viral stock of vAcBmT was then infected into Bombyx mori larvea, analysis showed that the TNF was expressed in it. After the TNF which expressed in Bombyx mori larvea was added into Hela cell, it induced the cell apoptosis. |
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