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Development of Optical Biosensor for the Detection of Glutamine in Human Biofluids Using Merocyanine Dye |
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| Authors: | Sathya Vijayakumar Deepa Appadurai Sangeetha Lakshmi Kandhan Srinivasadesikan Venkatesan Lee Shyi-Long Padmini Vediappen |
| Affiliation: | 1.Department of Organic Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625021, Tamilnadu, India ;2.Division of Chemistry, Department of Sciences and Humanities, Vignan’s Foundation for Science, Technology and Research, Vadlamudi, Guntur, 522 213, Andhra Pradesh, India ;3.Department of Chemistry and Biochemistry, National Chung-Cheng University, Chia-Yi, 621, Taiwan ; |
| Abstract: | Merocyanine dye based fluorescent organic compound has been synthesized for the detection of glutamine. The probe showed remarkable fluorescent intensity with glutamine through ICT (Intermolecular Charge Transfer Mechanism). Hence, it is tested for the detection of glutamine using colorimetric and fluorimetric techniques in physiological and neutral pH (7.2). Under optimized experimental conditions, the probe detects glutamine selectively among other interfering biomolecules. The probe has showed a LOD (lower limit of detection) of 9.6?×?10–8 mol/L at the linear range 0–180 µM towards glutamine. The practical application of the probe is successfully tested in human biofluids. Graphical abstract |
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