POCl3 catalyzed,one-step,solvent-free synthesis of some novel thieno[2,3-d]pyrimidin-4(3H)-one derivatives as antimicrobial agent

A POCl₃ catalyzed, efficient, one-step and solvent-free synthesis of novel thieno[2,3-d]pyrimidin-4(3H)-one derivatives from 2-amino-4,5-substitutedthiophene-3-carbonitrile has been developed under conventional heating and microwave irradiation. The formation of compounds was confirmed using elemental analysis and spectroscopic techniques like IR, NMR (¹H and ¹³C) and mass spectroscopy. Furthermore, they were screened in vitro to study their antimicrobial activity, which shows weak to moderate activity against all tested microorganisms.