Template free solvothermal synthesis of single crystal magnetic Fe3O4 hollow spheres,their interaction with bovine serum albumin and antibacterial activities

Uniform sized single crystal magnetic Fe₃O₄ hollow spheres (MFHS) have been synthesized through simple solvothermal method using ferric chloride hexahydrate and 1,3-propanediamine. The reaction time and the amount of 1,3-propanediamine play major roles in the formation of magnetic Fe₃O₄ hollow spheres. The synthesized products are characterized using X-ray diffraction, scanning electron microscopy, transmission electron microscopy and vibrating sample magnetometry techniques. The crystalline Fe₃O₄ materials are composed of well-aligned hollow sphere magnetite nanoparticles and exhibit high saturation magnetization of 57.9?emu/g and a remnant magnetization of 17.6?emu/g at room temperature. MFHS interact strongly with bovine serum albumin (BSA) and brings out considerable conformational changes in BSA as evident from the UV–vis absorption, fluorescence and circular dichroism (CD) spectroscopic studies pertaining to the interaction of synthesized MFHS and BSA. The prepared MFHS effectively inhibit the growth of Pseudomonas aeruginosa and Staphylococcus aureus.