| Theoretical study of the separation mechanism of ionizable compounds in capillary electrochromatography |
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| 引用本文： | 叶明亮,邹汉法,刘震,朱军,倪坚毅,张玉奎. Theoretical study of the separation mechanism of ionizable compounds in capillary electrochromatography[J]. 中国科学B辑(英文版), 1999, 0(6) |
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| 作者姓名： | 叶明亮 邹汉法 刘震 朱军 倪坚毅 张玉奎 |
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| 基金项目： | Project suppoted by the Excellent Young Scientist Award from the National Natural Science Foundation of China，the Natural Science Foundation of Liaoning Province. |
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| 摘 要： | The migration mechanism of ionizable compounds in capillary electrochromatography (CEC) is more complicated than in high performance liquid chromatography (HPLC) due to the involvement of electrophoresis and the second chemical equilibrium. The separation mechanism of ionizable compounds in CEC has been studied theoretically. The electrochromatographic capacity factors of ions (k~*) in CEC and in the pressurized CEC are derived by phenomenologicai approach. The influence of pH, voltage, pressure on k~* is discussed. In addition, the k~* of weak acid and weak base are derived based on acid-base equilibrium and the influence of pH on k~* is studied theoretically.
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Theoretical study of the separation mechanism of iomzable compounds in capillary electrochromatography |
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| YE Mingliang,ZOU Hanfa LIU ZhenZHU Jun Ni Jianyi and ZHANG Yukui. Theoretical study of the separation mechanism of iomzable compounds in capillary electrochromatography[J]. Science in China(Chemistry), 1999, 0(6) |
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| Authors: | YE Mingliang ZOU Hanfa LIU ZhenZHU Jun Ni Jianyi ZHANG Yukui |
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| Abstract: | The migration mechanism of ionizable compounds in capillary electrochromatography (CEC) is more complicated than in high performance liquid chromatography (HPLC) due to the involvement of electrophoresis and the second chemical equilibrium. The separation mechanism of ionizable compounds in CEC has been studied theoretically. The electrochromatographic capacity factors of ions ( k * ) in CEC and in the pressurized CEC are derived by phenomenologi-cal approach. The influence of pH, voltage, pressure on k * is discussed. In addition, the k * of weak acid and weak base are derived based on acid-base equilibrium and the influence of pH on k is studied theoretically. |
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| Keywords: | capillary electrochromatography pressurized capillary electrochromatography ionizable compounds separation mechanism. |
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