Protein recording material: photorecord/erasable protein array using a UV-eliminative linker |
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Authors: | Nakayama Koji Tachikawa Takashi Majima Tetsuro |
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Institution: | The Institute of Scientific and Industrial Research (ISIR), Osaka University Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan. |
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Abstract: | Protein patterning on solid surfaces is a topic of significant importance in the fields of biosensors, diagnostic assays, cell adhesion technologies, and biochip microarrays. In this letter, we have established a novel, rapid method for the fabrication of a "protein recording material", which enables us to spatiotemporally regulate the recording, reading, and erasing of a fluorescent protein array as information by a photochemical technique. A photolinker that we synthesized here was used to control the protein array spatiotemporally. The recording process was almost completed after 1 min of photoirradiation to read a clear pattern consisting of a specific protein-ligand complex with high spatiotemporal resolution. The erasing of the protein array was then achieved by photoirradiation onto the entire patterned surface. |
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