Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection |
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Authors: | Huang Ying Jiang Xiuyan Wang Wei Duan Jianping Chen Guonan |
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Institution: | a The MOE Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China b College of Chemistry and Materials Science, Fujian Normal University, Fuzhou, Fujian 350007, China |
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Abstract: | A method of capillary electrophoresis with wall-jet amperometric detection (AD) has been developed for separation and determination of l-tyrosine (Tyr) and its metabolites, such as Tyramine (TA), p-hydroxyphenylpyruvic (pHPP), homogentisic acid (HGA) and some dipeptides containing Tyr, such as Tyr-Gly-Gly (YGG), Tyr-Arg (YR) and Tyr-d-Arg (Y-d-R). A carbon disk electrode was used as the working electrode and the optimal detection potential was 1.00 V (versus Ag/AgCl). At 18 kV of applied voltage, the seven compounds were completely separated within 20 min in 110 × 10−3 mol/L Na2HPO4-NaH2PO4 buffer (pH 7.10) containing 3 × 10−3 mol/L β-cyclodextrin (β-CD). Good linear relationship was obtained for all analytes and the detection limits of seven analytes were in the range of 0.95-4.25 ng/mL. The proposed method has been applied to examine the metabolic process of l-tyrosine in rabbit's urine. |
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Keywords: | Capillary electrophoresis β-Cyclodextrin Amperometric detection Pharmacokinetics l-Tyrosine" target="_blank">l-Tyrosine |
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