Chitosan-based tyrosinase optical phenol biosensor employing hybrid nafion/sol-gel silicate for MBTH immobilization |
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Authors: | Abdullah Jaafar Ahmad Musa Heng Lee Yook Karuppiah Nadarajah Sidek Hamidah |
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Institution: | a School of Chemical Sciences and Food Technology, Faculty of Sciences and Technology, Universitiy Kebangsaan Malaysia, 43600 Bangi, Selangor D.E., Malaysia b Environmental and Bioprocess Technology Centre, SIRIM Berhad, No.1, Persiaran Dato’ Menteri, Section 2, P.O. Box 7035, 40911 Shah Alam, Selangor D.E., Malaysia |
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Abstract: | The development of an optical biosensor based on immobilization of 3-methyl-2-benzothiazolinone hydrazone (MBTH) in hybrid nafion/sol-gel silicate film and tyrosinase in chitosan film for the detection of phenolic compounds has been described. Tyrosinase was immobilized in chitosan film deposited on the hybrid nafion/sol-gel silicate film containing MBTH. The enzymatic oxidation product of phenolic compounds were stabilized through formation of adduct with MBTH to produce a maroon color adduct. The color intensity of adduct was found to increase proportionally with the increase of the substrate concentrations after 5 min exposure. The linearity of the biosensor towards phenol, catechol and m-cresol were in the respective concentration range of 0.5-7.0, 0.5-10.0 and 1.0-13.0 mg/L with detection limit of 0.18, 0.23 and 0.43 mg/L, respectively. The biosensor shows a good stability for at least 3 months. |
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Keywords: | Optical biosensor Tyrosinase Hybrid nafion/sol-gel silicate Chitosan Phenols |
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