Quantitative analysis of isoflavone aglycones in human serum by solid phase extraction and liquid chromatography-tandem mass spectrometry |
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Authors: | Chan Sun-An Lin Shi-Wey Yu Ken-Jen Liu Tsung-Yun Fuh Ming-Ren |
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Affiliation: | a Department of Medical Research and Education, Taipei Veterans General Hospital, Taiwan b Department of Chemistry, Soochow University, Taipei, Taiwan c Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, Taiwan |
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Abstract: | This paper describes a liquid chromatography-electrospray-tandem mass spectrometry (LC-ESI-MS/MS) for the qualitative and quantitative analysis of three isoflavone aglycones (glycitein, daidzein and genistein) in human serum. Positive ion mode was used for the detection of these compounds and selective reaction monitoring (SRM) was employed for quantitative measurement. The SRM transitions monitored were as 285.0 → 242.0, 270.0 for glycitein, 255.0 → 137.0, 153.0, 181.0, 199.0 for daidzein and 271.0 → 153.0, 215.0 for genistein. d3-Daidzein was used as an internal standard for quantitative measurement. The linearity was good from 0.5 to 500 ng/ml. The detection limit based on a signal-to-noise ratio of three was 0.27, 0.38 and 0.29 ng/ml for glycitein, daidzein and genistein, respectively. A newly developed solid phase extraction (SPE) procedure was developed for sample pre-treatment. Good recovery, 92.3-103.2%, for three isoflavone aglycones were obtained. This newly developed method was successfully applied to evaluate isoflavone pharmacokinetic in human serum after oral administration. |
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Keywords: | Isoflavone aglycones Liquid chromatography-tandem mass spectrometry Pharmacokinetic |
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