Simultaneous determination of tetracyclines in pharmaceuticals by CZE using experimental design |
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Authors: | Mamani Mónica Cecilia Vargas Farfán Jaime Amaya Reyes Felix Guillermo Reyes Rath Susanne |
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Institution: | a Institute of Chemistry, Department of Analytical Chemistry, State University of Campinas, P.O. Box 6154, 13084-971 Campinas, SP, Brazil b Department of Food & Nutrition, State University of Campinas, P.O. Box 6121, 13083-862 Campinas, SP, Brazil c Department of Food Science, State University of Campinas, P.O. Box 6154, 13083-970 Campinas, SP, Brazil |
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Abstract: | An optimized capillary zone electrophoresis (CZE) method for the analysis of tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC) and doxycycline (DXC) is described. Using fused-silica capillaries, the influence of the electrolyte composition, pH and concentration, as well as temperature and applied voltage were investigated. A factorial and central composite design was performed to optimize the method in a simple way. The optimal separation conditions were 50 mmol L−1 sodium carbonate + 1 mmol L−1 EDTA, pH 10; voltage 13 kV and temperature 23 °C. The method was validated for TC determination in pharmaceuticals through the following performance criteria: linearity and linear range, sensitivity, selectivity, intra-assay and inter-assay precision, detectability, accuracy and ruggedness. In comparison with the recommended HPLC method in the United States Pharmacopeia, this CZE-method exhibited the same performance as the official method, with the advantage that the same method could be used for the simultaneous determination of the different tetracyclines in pharmaceutical formulations. |
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Keywords: | Antibiotics Tetracyclines Capillary electrophoresis Validation |
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