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Complexes of the ATP-dependent Lon protease and DNA aptamers with G-quadruplexes as a model for developing a nanosensor biomagnetic immunoassay system |
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| Authors: | V A Spiridonova V A Sizov E O Kuzmenko A V Melnichuk E A Oleinichenko A M Kudzhaev T V Rotanova O V Snigirev |
| Institution: | 1.Belozersky Institute of Physicochemical Biology,Moscow State University,Moscow,Russia;2.Faculty of Chemistry,Moscow State University,Moscow,Russia;3.Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences,Moscow,Russia;4.Faculty of Physics,Moscow State University,Moscow,Russia |
| Abstract: | The binding to Lon protease through biotinylated aptamers whose structures contain G-quadruplex fragments with magnetic nanoparticles (MNPs) functionalized by streptavidin was investigated. The conditions of binding of target aptamers to MNPs are met. The resulting complexes are proposed for detection of Lon protease in different biological sources and for constructing a novel biomagnetic nanosensor immunoassay system. |
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