| 以交联聚乙烯醇为载体的离子交换剂对蛋白质的分离性能 |
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| 引用本文： | 许立忠,马建标.以交联聚乙烯醇为载体的离子交换剂对蛋白质的分离性能[J].离子交换与吸附,1996,12(2):114-118. |
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| 作者姓名： | 许立忠 马建标 |
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| 作者单位： | 南开大学高分子化学研究所，中国医学科学院血液学研究所 |
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| 摘 要： | 本文对阴离子交换剂ＤＥＡ—ＰＶＴ常压液相离子交换色谱分离蛋白质的性能、分离条件进行了探讨。结果表明其对蛋白质的分离性能良好，容易洗脱。与载体交联聚乙烯醇相比，ＤＥＡ—ＰＶＴ对蛋白质的非特异性吸附明显降低。
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| 关 键 词： | 阴离子交换剂，交联聚乙烯醇，蛋白质分离 |
SEPARATION OF SOME PROTEINS ON ANION EXCHANGE RESIN SUPPORTED BY CROSS-LINKED POLY(VINYL ALCOHOL) |
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| Xu Lizhong, Ma Jianbiao, He Binglin.SEPARATION OF SOME PROTEINS ON ANION EXCHANGE RESIN SUPPORTED BY CROSS-LINKED POLY(VINYL ALCOHOL)[J].Ion Exchange and Adsorption,1996,12(2):114-118. |
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| Authors: | Xu Lizhong Ma Jianbiao He Binglin |
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| Abstract: | The separation of proteins on an anion exchange resin (DEA -PVT) containing diethylamino groups supported by cross-linked poly(vinyl alcohol) was investigated under different conditions. The results showed that the proteins such as lysozyme, bovine serum albumin, cytochrome C, ovalbumin and hemoglobin were perfectly separated on this resin. Compared with its supportcross-linked poly(vinyl alcohol), the DEA-PVT possessed very little non-specific adsorption for proteins. |
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| Keywords: | anion exchange resin cross-linked poly(vinyl alcohol) separation of proteins |
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