| SDS-PAGE study on photooxidation damage of lysozyme induced by riboflavin |
| |
| 作者单位： | ZHANG ZhaoXia（Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;Graduate University of Chinese Academy of Sciences, Beijing 100049, China） ;ZHAO HongWei（Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China） ;ZHU HongPing（Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;Graduate University of Chinese Academy of Sciences, Beijing 100049, China） ;GE Min（Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;Graduate University of Chinese Academy of Sciences, Beijing 100049, China） ;WANG WenFeng（Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China） ;YAO SiDe （Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China） ;LI WenXin（Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China） ; |
| |
| 基金项目： | 国家自然科学基金;中国科学院资助项目 |
| |
| 摘 要： | The photooxidation damage of lysozyme under 315-375 nm irradiation in the presence of riboflavin was studied by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE).Indica- tions showed that the mechanisms and products of oxidative damage were relative to the concentra- tion of riboflavin,the time of irradiation and the ambience.The type I process was examined in a nitrogen saturated solution,whereas both type I and type II were observed in an aerobic atmosphere and type II was the dominant process.The study also suggested that antioxidants,such as melatonin, can reduce the damage of lysozyme effectively.
|
| 收稿时间： | 2006-01-21 |
| 修稿时间： | 2006-05-24 |
SDS-PAGE study on photooxidation damage of lysozyme induced by riboflavin |
| |
| Zhang ZhaoXia,Zhao HongWei,Zhu HongPing,Ge Min,Wang WenFeng,Yao SiDe,Li WenXin. SDS-PAGE study on photooxidation damage of lysozyme induced by riboflavin[J]. Science in China(Chemistry), 2007, 50(1): 84-90. DOI: 10.1007/s11426-007-0014-z |
| |
| Authors: | Zhang ZhaoXia Zhao HongWei Zhu HongPing Ge Min Wang WenFeng Yao SiDe Li WenXin |
| |
| Affiliation: | 1. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;Graduate University of Chinese Academy of Sciences, Beijing 100049, China
2. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China |
| |
| Abstract: | The photooxidation damage of lysozyme under 315-375 nm irradiation in the presence of riboflavin was studied by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE).Indica- tions showed that the mechanisms and products of oxidative damage were relative to the concentra- tion of riboflavin,the time of irradiation and the ambience.The type I process was examined in a nitrogen saturated solution,whereas both type I and type II were observed in an aerobic atmosphere and type II was the dominant process.The study also suggested that antioxidants,such as melatonin, can reduce the damage of lysozyme effectively. |
| |
| Keywords: | lysozyme riboflavin photooxidation SDS-PAGE antioxidant |
| 本文献已被 万方数据 SpringerLink 等数据库收录！ |
|
