| 金属硫蛋白MT-2a与Cd(Ⅱ)和Cu(Ⅰ)络合的电喷雾质谱表征 |
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| 引用本文： | 沈金灿,叶卫翔,康海宁,葛丽雅,庄峙厦,王小如.金属硫蛋白MT-2a与Cd(Ⅱ)和Cu(Ⅰ)络合的电喷雾质谱表征[J].分析化学,2009,37(7). |
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| 作者姓名： | 沈金灿 叶卫翔 康海宁 葛丽雅 庄峙厦 王小如 |
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| 作者单位： | 1. 深圳出入境检验检疫局食品检验与检疫技术中心,深圳,518067
2. 厦门大学化学化工学院,厦门,361005 |
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| 基金项目： | 国家自然科学基金，深圳出入境检验检疫局博士资金 |
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| 摘 要： | 采用电喷雾质谱法(ESI-MS)研究金属硫蛋白(MT,存在α和β两种结构域)-2a与金属离子Cd和Cu的络合作用.MT-2a由反相色谱分离纯化制得,并以电喷雾质谱鉴别.通过ESI-MS考察MT-2a与不同量的Cd和Cu的络合,结果表明,Cd能够优先与MT的α结构域络合,形成M2+4S11结构,并且该络合过程存在着明显的协同效应;Cd与MT的β结构域的络合形式为M2+4S9,其呈现出明显的随机松散络合特性;Cu优先与MT的β结构域络合,其络合形式由Cu4逐渐过渡到更高结合态;在Cu含量较高的条件下,Cu与MT呈现出多种络合方式.
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| 关 键 词： | 电喷雾质谱 金属硫蛋白 镉离子 铜离子 络合作用 |
Characterization of Cd and Cu Complexing with Metallothionein by Eletrospray Ionization Mass Spectrometry |
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| SHEN Jin-Can,YE Wei-Xiang,KANG Hai-Ning,GE Li-Ya,ZHUANG Zhi-Xia,WANG Xiao-Ru.Characterization of Cd and Cu Complexing with Metallothionein by Eletrospray Ionization Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2009,37(7). |
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| Authors: | SHEN Jin-Can YE Wei-Xiang KANG Hai-Ning GE Li-Ya ZHUANG Zhi-Xia WANG Xiao-Ru |
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| Institution: | SHEN Jin-Can1,YE Wei-Xiang1,KANG Hai-Ning1,GE Li-Ya1,ZHUANG Zhi-Xia2,WANG Xiao-Ru2 1(Inspection Center of Import and Export Food,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen 518067)2(Department of Chemistry and the Key Laboratory of Analytical Sciences of the Ministry of Education,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005) |
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| Abstract: | Mammal metallothionein(MT) folds into two separate domains that exhibit different structure and metal binding propertity independently, the study of the strategy of metal ions binding with MT would give better understanding of their exact biological functional mechanisms. In this study, a method using eletrospray ionization mass spectrometry (ESI-MS) phase liquid chromatography and identified by ESI-MS. Different amounts of Cd or Cu were then added in MT-2a samples and ESI-MS was employed to determine the mass difference of MT in different samples. The results Cd2+4S11; while Cd is attached in separate binding sites for the formation of Cd2+3S9 cluster, which intermediately formed with five and six Cd ions were detected. For the Cuprous ions, it prefers to cooperatively bind in β-domain with the form of Cu4-MTβ. The binding form in β-domain would convert from Cu4 into more Cu binding form with the addition of Cu. When high concentration of Cu was added in samples, the result suggested that |
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| Keywords: | Electrospray ionization mass spectrometry metallothionein |
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