Detection of carcinoembryonic antigen using functional magnetic and fluorescent nanoparticles in magnetic separators |
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Authors: | H Y Tsai C Y Chang Y C Li W C Chu K Viswanathan C Bor Fuh |
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Institution: | (1) Department of Applied Chemistry, Chung Shan Medical University, Taichung, 402, Taiwan;(2) Department of Applied Chemistry, National Chi Nan University, Puli, Nantou, 545, Taiwan;(3) Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, 402, Taiwan |
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Abstract: | We combined a sandwich immunoassay, anti-CEA/CEA/anti-CEA, with functional magnetic (~80 nm) and fluorescent (~180 nm) nanoparticles
in magnetic separators to demonstrate a detection method for carcinoembryonic antigen (CEA). Determination of CEA in serum
can be used in clinical diagnosis and monitoring of tumor-related diseases. The CEA concentrations in samples were deduced
and determined based on the reference plot using the measured fluorescent intensity of sandwich nanoparticles from the sample.
The linear range of CEA detection was from 18 ng/mL to 1.8 pg/mL. The detection limit of CEA was 1.8 pg/mL. In comparison
with most other detection methods, this method had advantages of lower detection limit and wider linear range. The recovery
was higher than 94%. The CEA concentrations of two serum samples were determined to be 9.0 and 55 ng/mL, which differed by
6.7% (9.6 ng/mL) and 9.1% (50 ng/mL) from the measurements of enzyme-linked immunosorbent assay (ELISA), respectively. The
analysis time can be reduced to one third of ELISA. This method has good potential for other biomarker detections and biochemical
applications. |
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