Study on the interactions between anti-cancer drug oxaliplatin and DNA by atomic force microscopy |
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| Institution: | 1. Laboratory of Enzymology and Protein Folding, Centre for Protein Engineering, University of Liege, Liege, Belgium;2. Nanochemistry and Molecular Systems, Department of Chemistry, University of Liege, Liege, Belgium;3. Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK;4. Center for Education and Research on Macromolecules (CERM), Department of Chemistry, University of Liege, Liege, Belgium;1. Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, CZ-61265 Brno, Czech Republic;2. Central European Institute of Technology, Masaryk University, Kamenice 753/5, CZ-62500 Brno, Czech Republic;3. Charles University, Faculty of Science, University Research Centre UNCE “Supramolecular Chemistry”, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Prague, Hlavova 2030/8, CZ-12843 Prague 2, Czech Republic |
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| Abstract: | Oxaliplatin is one of the most important anticancer drugs at present. However, the mechanism of action of oxaliplatin is still controversial. In this study, the interactions between oxaliplatin and a plasmid DNA have been studied so as to reveal the structural basis of its activity. The structural characteristic of pUC19 DNA (2 ng/μL) incubated with 100 μmol/L and 1000 μmol/L of oxaliplatin for the different time on a freshly cleaved highly ordered pyrolytic graphite (HOPG) surface was investigated by atomic force microscopy (AFM). High resolution AFM observation indicated that oxaliplatin can induce pUC19 DNA molecules change from the extended conformation to the entangled structures with many nodes, and finally to the compact particles. The present AFM results provide structural evidence about the interactions between oxaliplatin and circular duplex DNA containing multiple targets. |
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| Keywords: | Oxaliplatin DNA HOPG Atomic force microscopy AFM |
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