The evidence of Tobacco rattle virus impact on host plant organelles ultrastructure |
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| Institution: | 1. Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, WULS-SGGW, Nowoursynowska Str. 159, 02-776 Warsaw, Poland;2. Plant Molecular Biology Center and the Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA;3. Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61-704 Poznan, Poland;1. Instituto de Ciencia de Materiales de Sevilla (CSIC), 41092 Sevilla, Spain;2. Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons and Peter Grünberg Institute, Forschungszentrum Jülich, D-52425 Jülich, Germany;1. Department of Plant Biology, College of Biological Sciences, University of California, Davis, CA 95616, USA;2. Department of Plant Pathology, National Chung-Hsing University, Taichung;1. Emerging Pest and Pathogen Research Unit, USDA, ARS and Section of Plant Pathology and Plant-Microbe Biology, School of Integrated Plant Science, Cornell University, Ithaca, NY 14853, United States;2. Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY 14853, United States;1. Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China;2. University of the Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Tobraviruses, like other (+) stranded RNA viruses of plants, replicate their genome in cytoplasm and use such usual membranous structures like endoplasmic reticulum. Based on the ultrastructural examination of Tobacco rattle virus (TRV)-infected potato and tobacco leaf tissues, in this work we provide evidence of the participation of not only the membranous and vesicular ER structures but also other cell organelles during the viral infection cycle. Non-capsidated TRV PSG particles (potato isolate from the Netherlands) (long and short forms) were observed inside the nucleus while the presence of TRV capsid protein (CP) was detected in the nucleus caryolymph and within the nucleolus area. Both capsidated and non-capsidated viral particles were localized inside the strongly disorganized chloroplasts and mitochondria. The electron-dense TRV particles were connected with vesicular structures of mitochondria as well as with chloroplasts in both potato and tobacco tissues. At 15–30 days after infection, vesicles filled with TRV short particles were visible in mitochondria revealing the expanded cristae structures. Immunodetection analysis revealed the TRV PSG CP epitope inside chloroplast with disorganized thylakoids structure as well as in mitochondria of different tobacco and potato tissues. The ultrastructural analysis demonstrated high dynamics of the main cell organelles during the TRV PSG–Solanaceous plants interactions. Moreover, our results suggest a relationship between organelle changes and different stages of virus infection cycle and/or particle formation. |
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| Keywords: | Chloroplast Endoplasmic reticulum Mitochondria Nucleus Plant–virus interaction Tobacco rattle virus |
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