Purification of a marine bacterial glucose dehydrogenase fromCytophaga marinoflava and its application for measurement of 1,5-anhydro-d-glucitol |
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| Authors: | Wakako Tsugawa Shuichi Horiuchi Mitsuharu Tanaka Hitoshi Wake Koji Sode |
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| Affiliation: | (1) Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, 184 Koganei Tokyo, Japan;(2) Central Research Laboratory, Pentel Co., Ltd., 4-1-8 Soka-City, 340 Saitama, Japan |
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| Abstract: | A novel glucose dehydrogenase (GDH) from a marine bacteriumCytophaga marinoflava IFO 14170 was isolated from its membrane fraction. This GDH catalyzes the oxidation of a hydroxy group of glucose, but does not react in its C-l position. This enzyme is composed of a single peptide with a mol wt of 67,000. The GDH can react under high salinity. The optimum pH is around 8.0, showing a typical property of marine bacterial enzymes. Using this novel enzyme, an enzymatic determination of 1,5-anhydro-D-glucitol (1,5AG) utilizing 2,6-dichrolophenolindophenol (DCIP) and phenazine methosulfate (PMS) as electron mediators was caried out. A good linear correlation was observed from 0.5 mM to 4 mM of 1,5AG. |
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| Keywords: | Glucose dehydrogenase Marine bacteria 1,5-Anhydro-D-glucitol diabetes Cytophaga marinoflava |
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