A Method for Evaluating the Level of Soluble β‐Amyloid(1–40/1–42) in Alzheimer’s Disease Based on the Binding of Gelsolin to β‐Amyloid Peptides |
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| Authors: | Dr. Yanyan Yu Lin Zhang Dr. Chenglin Li Xiaoyu Sun Dr. Daoquan Tang Prof. Guoyue Shi |
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| Affiliation: | 1. Department of Pharmaceutical Analysis, Xuzhou Medical College, Xuzhou 221004 (China);2. Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou 221004 (China);3. Department of Chemistry, East China Normal University, 500 Dongchuan Road, Shanghai 200241 (China) |
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| Abstract: | In the present work, a new electrochemical strategy for the sensitive and specific detection of soluble β‐amyloid Aβ(1–40/1–42) peptides in a rat model of Alzheimer’s disease (AD) is described. In contrast to previous antibody‐based methods, β‐amyloid(1–40/1–42) was quantified based on its binding to gelsolin, a secretory protein present in the cerebrospinal fluid (CSF) and plasma. The level of soluble β‐amyloid peptides in the CSF and various brain regions were found with this method to be lower in rats with AD than in normal rats. |
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| Keywords: | aggregation Alzheimer’ s disease β ‐amyloid peptides biosensors electrochemistry |
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