Photodegradable Hydrogels for Capture,Detection, and Release of Live Cells |
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| Authors: | Dr Dong‐Sik Shin Dr Jungmok You Ali Rahimian Tam Vu Christian Siltanen Arshia Ehsanipour Dr Gulnaz Stybayeva Prof Julie Sutcliffe Prof Alexander Revzin |
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| Institution: | 1. Department of Biomedical Engineering, University of California Davis, One Shields Ave, Davis, CA 95616 (USA);2. Division of Hematology/Oncology, Department of Internal Medicine, Center for Molecular and Genomic Imaging, University of California, Davis, Davis, CA 95616 (USA) |
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| Abstract: | Cells may be captured and released using a photodegradable hydrogel (photogel) functionalized with antibodies. Photogel substrates were used to first isolate human CD4 or CD8 T‐cells from a heterogeneous cell suspension and then to release desired cells or groups of cells by UV‐induced photodegradation. Flow cytometry analysis of the retrieved cells revealed approximately 95 % purity of CD4 and CD8 T‐cells, suggesting that this substrate had excellent specificity. To demonstrate the possibility of sorting cells according to their function, photogel substrates that were functionalized with anti‐CD4 and anti‐TNF‐α antibodies were prepared. Single cells captured and stimulated on such substrates were identified by the fluorescence “halo” after immunofluorescent staining and could be retrieved by site‐specific exposure to UV light through a microscope objective. Overall, it was demonstrated that functional photodegradable hydrogels enable the capture, analysis, and sorting of live cells. |
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| Keywords: | antibodies microarrays micropatterning hydrogels single‐cell detection |
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